| Reference | Liters (L) | Microliters (µL) |
|---|---|---|
| A teaspoon | 0.005 L | 5000 µL |
| A can of soft drink | 0.33 L | 330000 µL |
| A wine bottle | 0.75 L | 750000 µL |
| A bathtub | 150 L | 150000000 µL |
| An Olympic swimming pool | 2500000 L | 2.5 × 1012 µL |
The litre is a unit of volume equal to one thousandth of a cubic metre, or exactly one cubic decimetre. Its symbol is L, and the lower-case l is also permitted. It is not part of the International System of Units, but it is accepted for use alongside it, and it is almost certainly the most widely used volume unit in the world.
It was created by the French metric law of 1795 as the volume of a cube one tenth of a metre on a side. In 1901 it was redefined as the volume occupied by one kilogram of pure water at its densest, which sounds equivalent but is not: the water definition made a litre 1.000028 cubic decimetres, and for sixty-three years precise volumetric work had to state which litre was meant. The 1964 General Conference on Weights and Measures restored the cubic decimetre and the discrepancy vanished.
The symbol has its own small history. The lower-case l is easily mistaken for the digit one in many typefaces, a genuine hazard on a medicine label or a laboratory record, so in 1979 the capital L was approved as an alternative. Both are correct, and usage splits by country rather than by discipline, with North America favouring L and much of Europe still writing l.
Its everyday reach is enormous. Fuel, milk, paint, soft drinks and engine displacement are all measured in litres, fuel consumption in most of the world is stated as litres per hundred kilometres, and cooking outside the United States works in litres and millilitres. Medicine uses it for blood volume, roughly five litres in an adult, and for lung capacity, around six.
One relationship makes it exceptionally useful. A litre of water has a mass very close to one kilogram, because the kilogram was originally defined that way, so volume and mass can be swapped in the head for most practical purposes. Rainfall follows the same logic: one millimetre of rain on one square metre is exactly one litre of water.
Against imperial and United States measures it converts untidily, which is why drink sizes differ so visibly between markets. One litre is 1.76 imperial pints but 2.11 United States liquid pints, and 0.22 imperial gallons but 0.264 United States gallons, so a gallon means two noticeably different quantities depending on where the label was printed.
One litre equals 1000 millilitres, 0.001 cubic metres, about 0.264 US gallons, or about 0.22 imperial gallons.
The microlitre is a unit of volume equal to one millionth of a litre, written µL. It is exactly one cubic millimetre. It is the working unit of the molecular biology laboratory and of clinical haematology, and almost every quantity in modern biological science that is not a mass or a concentration is a number of microlitres.
Pipetting is what defines it in practice. Adjustable micropipettes are named after their maximum volume in microlitres, so a bench holds instruments of 2, 20, 200 and 1000, and a researcher's day consists largely of moving microlitres from one tube to another. A polymerase chain reaction is typically assembled in twenty to fifty microlitres, and a whole experiment may use less liquid than a teaspoon holds.
Blood counts are reported against it. White cells number between four thousand and eleven thousand per microlitre in a healthy adult, platelets between a hundred and fifty thousand and four hundred thousand, and red cells about five million. Those reference ranges are among the most widely used numbers in medicine, and all of them are counts within a cube one millimetre on a side.
Diagnostics has driven the volumes down. A modern blood glucose meter needs about half a microlitre of blood, which is why a finger-prick sample is enough where an earlier generation of instruments needed a syringe. Point-of-care analysers, lateral flow tests and microfluidic cartridges are all designed around the smallest sample a patient can be asked to give.
Analytical chemistry uses the same scale. Injection volumes in liquid chromatography run from one to twenty microlitres, gas chromatography uses one or less, and mass spectrometry samples are prepared in tens. Because the analytical signal depends on the amount injected, the accuracy of a microlitre-scale measurement sets the accuracy of the whole result.
The identity with the cubic millimetre is a genuine convenience. Haematology once reported cell counts per cubic millimetre and now reports them per microlitre, and the numbers did not change at all, because the two units are the same. That is a rare case of a unit change in medicine that required no re-education and produced no errors.
One microlitre equals 1 cubic millimetre, 0.001 millilitres, 0.000001 litres, or about 0.0000338 US fluid ounces.