| Reference | Liters (L) | Nanoliters (nL) |
|---|---|---|
| A teaspoon | 0.005 L | 5000000 nL |
| A can of soft drink | 0.33 L | 330000000 nL |
| A wine bottle | 0.75 L | 750000000 nL |
| A bathtub | 150 L | 1.5 × 1011 nL |
| An Olympic swimming pool | 2500000 L | 2.5 × 1015 nL |
The litre is a unit of volume equal to one thousandth of a cubic metre, or exactly one cubic decimetre. Its symbol is L, and the lower-case l is also permitted. It is not part of the International System of Units, but it is accepted for use alongside it, and it is almost certainly the most widely used volume unit in the world.
It was created by the French metric law of 1795 as the volume of a cube one tenth of a metre on a side. In 1901 it was redefined as the volume occupied by one kilogram of pure water at its densest, which sounds equivalent but is not: the water definition made a litre 1.000028 cubic decimetres, and for sixty-three years precise volumetric work had to state which litre was meant. The 1964 General Conference on Weights and Measures restored the cubic decimetre and the discrepancy vanished.
The symbol has its own small history. The lower-case l is easily mistaken for the digit one in many typefaces, a genuine hazard on a medicine label or a laboratory record, so in 1979 the capital L was approved as an alternative. Both are correct, and usage splits by country rather than by discipline, with North America favouring L and much of Europe still writing l.
Its everyday reach is enormous. Fuel, milk, paint, soft drinks and engine displacement are all measured in litres, fuel consumption in most of the world is stated as litres per hundred kilometres, and cooking outside the United States works in litres and millilitres. Medicine uses it for blood volume, roughly five litres in an adult, and for lung capacity, around six.
One relationship makes it exceptionally useful. A litre of water has a mass very close to one kilogram, because the kilogram was originally defined that way, so volume and mass can be swapped in the head for most practical purposes. Rainfall follows the same logic: one millimetre of rain on one square metre is exactly one litre of water.
Against imperial and United States measures it converts untidily, which is why drink sizes differ so visibly between markets. One litre is 1.76 imperial pints but 2.11 United States liquid pints, and 0.22 imperial gallons but 0.264 United States gallons, so a gallon means two noticeably different quantities depending on where the label was printed.
One litre equals 1000 millilitres, 0.001 cubic metres, about 0.264 US gallons, or about 0.22 imperial gallons.
The nanolitre is a unit of volume equal to one billionth of a litre, written nL. It is a thousandth of a microlitre, and a nanolitre of water has a mass of one microgram. It is the scale at which liquid handling stops being a matter of pipettes and becomes a matter of engineered channels, printed droplets and acoustic pulses.
Microfluidics is built here. A lab-on-a-chip moves samples through channels a few tens of micrometres wide, so the volume inside any one segment is measured in nanolitres, and a whole analysis may consume less than a microlitre. Working at this scale changes the physics as well as the quantity: surface tension dominates gravity, flows stay orderly rather than turbulent, and mixing has to be engineered rather than assumed.
Digital polymerase chain reaction shows what the scale buys. A sample is divided into around twenty thousand droplets of roughly one nanolitre each, every droplet is amplified separately, and the number that come out positive gives an absolute count of target molecules rather than a relative measurement. Dividing a microlitre into thousands of countable compartments is only possible because a nanolitre is small enough to hold either one target molecule or none.
Drug screening moves liquid without touching it. Acoustic dispensers use a focused sound pulse to eject droplets of two and a half nanolitres from an open well, with no tip and therefore no cross-contamination and no plastic waste. A screening plate of fifteen hundred wells can be filled with distinct compounds in a few minutes at this volume.
Printing and spotting work at the same magnitude. A microarray spot holds between half a nanolitre and five, an inkjet droplet is a few picolitres so a nanolitre is several hundred drops, and single-cell platforms encapsulate individual cells in droplets of this size because a mammalian cell occupies only a few picolitres.
Handling nanolitres reliably is mostly a fight against evaporation. A one-nanolitre droplet exposed to air can lose a measurable fraction of itself in seconds, so devices at this scale work under oil, in sealed channels or in humidity-controlled enclosures, and that constraint shapes the instruments more than the dispensing itself does.
One nanolitre equals 0.001 microlitres, 0.000001 millilitres, 0.001 cubic millimetres, or 1,000,000 cubic micrometres.