| Nanoliters (nL) | Milliliters (mL) |
|---|---|
| 1 Nanoliter | 0.000001 mL |
| 2 Nanoliters | 0.000002 mL |
| 3 Nanoliters | 0.000003 mL |
| 4 Nanoliters | 0.000004 mL |
| 5 Nanoliters | 0.000005 mL |
| 10 Nanoliters | 0.00001 mL |
| 20 Nanoliters | 0.00002 mL |
| 25 Nanoliters | 0.000025 mL |
| 50 Nanoliters | 0.00005 mL |
| 100 Nanoliters | 0.0001 mL |
| Reference | Nanoliters (nL) | Milliliters (mL) |
|---|---|---|
| A teaspoon | 5000000 nL | 5 mL |
| A can of soft drink | 330000000 nL | 330 mL |
| A wine bottle | 750000000 nL | 750 mL |
| A bathtub | 1.5 × 1011 nL | 150000 mL |
| An Olympic swimming pool | 2.5 × 1015 nL | 2.5 × 109 mL |
The nanolitre is a unit of volume equal to one billionth of a litre, written nL. It is a thousandth of a microlitre, and a nanolitre of water has a mass of one microgram. It is the scale at which liquid handling stops being a matter of pipettes and becomes a matter of engineered channels, printed droplets and acoustic pulses.
Microfluidics is built here. A lab-on-a-chip moves samples through channels a few tens of micrometres wide, so the volume inside any one segment is measured in nanolitres, and a whole analysis may consume less than a microlitre. Working at this scale changes the physics as well as the quantity: surface tension dominates gravity, flows stay orderly rather than turbulent, and mixing has to be engineered rather than assumed.
Digital polymerase chain reaction shows what the scale buys. A sample is divided into around twenty thousand droplets of roughly one nanolitre each, every droplet is amplified separately, and the number that come out positive gives an absolute count of target molecules rather than a relative measurement. Dividing a microlitre into thousands of countable compartments is only possible because a nanolitre is small enough to hold either one target molecule or none.
Drug screening moves liquid without touching it. Acoustic dispensers use a focused sound pulse to eject droplets of two and a half nanolitres from an open well, with no tip and therefore no cross-contamination and no plastic waste. A screening plate of fifteen hundred wells can be filled with distinct compounds in a few minutes at this volume.
Printing and spotting work at the same magnitude. A microarray spot holds between half a nanolitre and five, an inkjet droplet is a few picolitres so a nanolitre is several hundred drops, and single-cell platforms encapsulate individual cells in droplets of this size because a mammalian cell occupies only a few picolitres.
Handling nanolitres reliably is mostly a fight against evaporation. A one-nanolitre droplet exposed to air can lose a measurable fraction of itself in seconds, so devices at this scale work under oil, in sealed channels or in humidity-controlled enclosures, and that constraint shapes the instruments more than the dispensing itself does.
One nanolitre equals 0.001 microlitres, 0.000001 millilitres, 0.001 cubic millimetres, or 1,000,000 cubic micrometres.
The millilitre is a unit of volume equal to one thousandth of a litre, written mL or ml. It is exactly one cubic centimetre. It is the working unit of medicine, cookery, cosmetics and the laboratory, and it is the smallest volume most people handle deliberately in the course of a day.
Medicine relies on it more heavily than on any other unit of volume. Oral liquids are dosed in millilitres, syringes are graduated in them, intravenous fluids are prescribed as so many millilitres per hour, and blood test results are reported per millilitre or per decilitre. The precision matters: a tenfold dosing error in a paediatric prescription is a difference of a single decimal place in this unit.
That is why the household spoon has been driven out of medical instructions. A teaspoon is nominally five millilitres, but real spoons vary from two to nine, so dosing devices are now supplied with liquid medicines and marked in millilitres. Regulators in several countries require the millilitre marking precisely because it removes an ambiguity that spoons cannot.
Cooking outside the United States runs on it. European and Asian recipes give liquids in millilitres and litres rather than in cups, which is more precise because a cup measures volume by an unstandardised container while a millilitre does not. Wine and spirit measures, coffee shot sizes, and the capacity of a baby bottle are all specified in millilitres.
A convenient identity helps in the kitchen and the laboratory alike. One millilitre of water has a mass of one gram, so a recipe or a protocol can move between mass and volume for water-like liquids without a real error. Cosmetics use the same unit throughout, from a five-millilitre sample to a five-hundred-millilitre bottle.
Against United States and imperial measures it converts awkwardly, and the two differ from each other. One millilitre is 0.0338 United States fluid ounces but 0.0352 imperial fluid ounces, so a fluid ounce is about four per cent larger in Britain than in America, and any drink or medicine volume converted between them needs to state which system was meant.
One millilitre equals 1 cubic centimetre, 0.001 litres, about 0.0338 US fluid ounces, or about 0.0352 imperial fluid ounces.