Microliter (µL)

The microlitre is a unit of volume equal to one millionth of a litre, written µL. It is exactly one cubic millimetre. It is the working unit of the molecular biology laboratory and of clinical haematology, and almost every quantity in modern biological science that is not a mass or a concentration is a number of microlitres.

Pipetting is what defines it in practice. Adjustable micropipettes are named after their maximum volume in microlitres, so a bench holds instruments of 2, 20, 200 and 1000, and a researcher's day consists largely of moving microlitres from one tube to another. A polymerase chain reaction is typically assembled in twenty to fifty microlitres, and a whole experiment may use less liquid than a teaspoon holds.

Blood counts are reported against it. White cells number between four thousand and eleven thousand per microlitre in a healthy adult, platelets between a hundred and fifty thousand and four hundred thousand, and red cells about five million. Those reference ranges are among the most widely used numbers in medicine, and all of them are counts within a cube one millimetre on a side.

Diagnostics has driven the volumes down. A modern blood glucose meter needs about half a microlitre of blood, which is why a finger-prick sample is enough where an earlier generation of instruments needed a syringe. Point-of-care analysers, lateral flow tests and microfluidic cartridges are all designed around the smallest sample a patient can be asked to give.

Analytical chemistry uses the same scale. Injection volumes in liquid chromatography run from one to twenty microlitres, gas chromatography uses one or less, and mass spectrometry samples are prepared in tens. Because the analytical signal depends on the amount injected, the accuracy of a microlitre-scale measurement sets the accuracy of the whole result.

The identity with the cubic millimetre is a genuine convenience. Haematology once reported cell counts per cubic millimetre and now reports them per microlitre, and the numbers did not change at all, because the two units are the same. That is a rare case of a unit change in medicine that required no re-education and produced no errors.

One microlitre equals 1 cubic millimetre, 0.001 millilitres, 0.000001 litres, or about 0.0000338 US fluid ounces.

Microliter conversion table